Skip to main content
. 2023 Feb 9;24(4):3477. doi: 10.3390/ijms24043477

Figure 3.

Figure 3

The effect of EV treatment on neuronal populations in trisomic CS. (A) Single and merged channels of confocal images showing DAPI staining with IHC labeling of TBR1 (magenta), CTIP2 (green) and SATB2 (red) in euploid and trisomic untreated and EV-treated CS generated from isogenic cell lines. Scale bar = 100 µm. (B–D) Bar graphs showing quantification of percentages of SATB2+, CTIP2+, and TBR1+ cells in euploid CS and trisomic untreated and EV-treated CS, quantified as the ratio of the numbers of CTIP2+, SATB2+, and TBR1+ to the number of all cells marked with DAPI and normalized to the euploid control that was calculated as 100% for each independent experiment. Cells were counted using the ACEq counting app; euploid n = 18; trisomic n = 22; trisomic EV+ n = 22 CS per group. These graphs reflect the results obtained from data collected from three independent experiments. Statistical analysis performed via unpaired t-test with Welch’s correction comparing all three groups to each other. Results are shown as mean ± SE. * p < 0.05, *** p < 0.001, **** p < 0.0001, ns = non-significant.