Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 14;222(4):e202204093. doi: 10.1083/jcb.202204093

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Barad et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Distance between IMMs and OMMs is dependent on mitochondrial network morphology and presence or absence of ER stress. (A) Surface membrane reconstruction defining OMM (purple) and IMM (orange) distance measurement. (B) Representative membrane surface reconstructions of elongated (top) and fragmented (bottom) mitochondria in MEF^mtGFP cells treated with vehicle and Tg (500 nM, 8 h). The OMM surface is colored by outer-to-inner (OMM–IMM) membrane distance, and the IMM surface is shown in transparent gray. Red arrows indicate regions on OMM with large OMM–IMM distances that correspond to cristae junctions. (C) Combined histogram of OMM–IMM distances of elongated (top) and fragmented (bottom) mitochondria in MEF^mtGFP cells treated with vehicle and Tg. Dashed vertical lines correspond to peak histogram values of pooled data. (D) Quantification of peak histogram values from each mitochondrion within the indicated treatment and mitochondrial morphology class. Quantifications from vehicle elongated n = 20, Tg elongated n = 18, vehicle fragmented n = 11, and Tg fragmented n = 15 mitochondria are shown. P values from Mann–Whitney U test are indicated. *P < 0.05; ****P < 0.001.