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. 2023 Feb 14;222(4):e202204093. doi: 10.1083/jcb.202204093

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© 2023 Barad et al.

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Figure 5. — The curvedness of mitochondrial cristae and crista junctions are dependent both on mitochondrial network morphology and Tg-induced ER stress. (A) Representative membrane surface reconstructions of elongated (top) and fragmented (bottom) mitochondria in MEF^mtGFP cells treated with vehicle and Tg (500 nM, 8 h). The IMM surface is colored by membrane curvedness and the OMM surface is represented as a transparent gray mesh. Curvedness is an unsigned combination of the two principal components of curvedness and is used because the surface normal vectors do not have canonical sign. (B) Combined histogram of total IMM curvedness of elongated (top) and fragmented (middle) mitochondria in MEF^mtGFP cells treated with vehicle and Tg. Dashed vertical lines correspond to peak histogram values of pooled data. Graph (bottom) showing peak histogram values from each mitochondrion within each corresponding treatment and mitochondrial morphology class. Quantifications from vehicle elongated n = 20, Tg elongated n = 18, vehicle fragmented n = 11, and Tg fragmented n = 15 mitochondria are shown. P values from Mann–Whitney U test are indicated. **P < 0.01. (C) Combined histogram of cristae curvedness of elongated (left) and fragmented (middle) mitochondria in MEF^mtGFP cells treated with vehicle and Tg. Dashed vertical lines correspond to peak histogram values of pooled data. Graph (right) showing peak histogram values from each mitochondrion within each corresponding treatment and mitochondrial morphology class. Quantifications from vehicle elongated n = 20, Tg elongated n = 18, vehicle fragmented n = 11, and Tg fragmented n = 15 mitochondria are shown. P values from Mann–Whitney U test are indicated. *P < 0.05; **P < 0.01. (D) Combined histogram of cristae junction curvedness of elongated (left) and fragmented (middle) mitochondria in MEF^mtGFP cells treated with vehicle and Tg. Dashed vertical lines correspond to peak histogram values of pooled data. Graph (right) showing peak histogram values from each mitochondrion within each corresponding treatment and mitochondrial morphology class. Quantifications from vehicle elongated n = 20, Tg elongated n = 18, vehicle fragmented n = 11, and Tg fragmented n = 15 mitochondria are shown. P values from Mann–Whitney U test are indicated. *P < 0.05; ****P < 0.001.