Figure S1.

Gallery of cellular membranes used for downstream quantifications from cells with elongated mitochondrial networks in the absence of ER stress. Virtual slices of tomograms (upper panels) and corresponding reconstructed surface mesh models (lower panels) of mitochondrial membranes (IMM, orange; OMM, purple) and ER membranes (blue). The surfaces (lower panels) are labeled according to treatment conditions (U, untreated/vehicle-treated), mitochondrial morphology (E, elongated), and tomogram number. For example, “UE1” is untreated, elongated, tomogram 1. Individual mitochondria in the tomograms are labeled by letters (e.g., a, b, c). These labels correspond to the title labels of the histograms of individual mitochondria shown in Fig. S5. Meshes have been tilted backward by 20° for visualization. Scale bar = 100 nm. Vehicle-treated cells with elongated mitochondrial networks, n = 10 tomograms.