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. 2023 Feb 14;222(4):e202204093. doi: 10.1083/jcb.202204093

Video 1.

Comparison of voxel segmentation vs. surface reconstruction. An example tomogram is shown with and without a completed voxel segmentation (green). A zoom in reveals voxel stepping artifacts that impact measurement precision, as well as small holes that result from automated segmentation. Switching to the reconstructed surface shows the smooth triangle mesh and the hole-filling properties of the surface reconstruction algorithm. Surface colors: IMM in pink, OMM in purple, ER in blue.