Table 3.

Experimental plan based on central composite design.

Run	Homogenisation Time (min)	Ultrasonication Time (min)	Amplitude (%)
1	5	10	50
2	10	10	50
3	5	20	50
4	10	20	50
5	5	10	70
6	10	10	70
7	5	20	70
8	10	20	70
9	5	15	60
10	10	15	60
11	7.5	10	60
12	7.5	20	60
13	7.5	15	50
14	7.5	15	70
15	7.5	15	60
16	7.5	15	60
17	7.5	15	60