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. 2022 Apr 12;62(1):347–359. doi: 10.1093/rheumatology/keac227

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Fig. 4 — Cytokine secretion after monocyte stimulation

Representative example of two healthy controls and P1 is depicted. (A) Comparison of cytokine production by adherent (monocytes) and non-adherent (lymphocytes) fractions from PBMCs after stimulation with standard LPS (stimulates through TLR2 and TLR4) and ultrapure LPS (stimulates through TLR4). (B) After stimulation, P1 shows maximal IL-6 comparable to the healthy control [HC]. (C) TNF-α production was highest in P1. The average IL-6 and TNF-α levels in eight healthy controls after 24 h stimulation with 100 ng/mL of ultrapure LPS is 9410 (4476) pg/mL and 261 (149) pg/mL, respectively.