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Enhancement of tubulin polymerization by Breastin. Tubulin was incubated with DMSO (negative control), Breastin (13.2 and 132 µg/mL), and paclitaxel (10 µM) at 37 °C. Optical density was determined every 30 s for 3 h. A shift to the upper left compared to DMSO reflected an increase in microtubule polymerization.
