Table 1. Summary of effects of loss of FXR on NM-induced lung histopathology.
Sections were prepared 3 d, 14 d and 28 d after administration of PBS (CTL) or nitrogen mustard (NM) to male wild-type (WT) and FXR−/− mice. Histopathologic changes were quantified in H&E and Masson’s trichrome stained sections as described in the Materials and Methods. Values are mean ± SE, n = 4–5 mice/treatment/time point.
| Histopathology | WT |
FXR−/− |
|||
|---|---|---|---|---|---|
| CTL | NM | CTL | NM | ||
| 3 d | Epithelial hyperplasia | – | – | – | – |
| Perivascular inflammation | – | 1.4 ± 0.2 | – | 1.75 ± 0.3 | |
| Perivascular edema | – | 1.4 ± 0.2 | – | 2.0 ± 0.0# | |
| Neutrophils (alveolar) | – | 1.0 ± 0.3 | – | 1.5 ± 0.3 | |
| Foamy alveolar macrophages | – | – | – | – | |
| Alveolar wall thickening | – | – | – | – | |
| Fibrosis | – | 0.4 ± 0.2 | – | 0.5 ± 0.3 | |
| 14 d | Epithelial hyperplasia | – | 0.2 ± 0.2 | – | 0.8 ± 0.2# |
| Perivascular inflammation | – | 0.8 ± 0.2 | – | 1.2 ± 0.4 | |
| Perivascular edema | – | – | – | 0.8 ± 0.4 | |
| Neutrophils (alveolar) | – | 0.4 ± 0.2 | – | 0.8 ± 0.2 | |
| Foamy alveolar macrophages | – | 1.4 ± 0.2 | – | 1.8 ± 0.2 | |
| Alveolar wall thickening | – | 1.6 ± 0.7 | – | 3.8 ± 0.7# | |
| Fibrosis | 0.2 ± 0.2 | 1.0 ± 0.3 | 0.2 ± 0.2 | 2.2 ± 0.4*# | |
| 28 d | Epithelial hyperplasia | – | – | – | – |
| Perivascular inflammation | – | 1.0 ± 0.0 | 0.2 ± 0.0 | 1.0 ± 0.0 | |
| Perivascular edema | – | – | – | – | |
| Neutrophils (alveolar) | – | – | – | – | |
| Foamy alveolar macrophages | – | 1.0 ± 0.0 | – | 1.0 ± 0.0 | |
| Alveolar wall thickening | – | 1.0 ± 0.0 | – | 1.0 ± 0.0 | |
| Fibrosis | – | 1.0 ± 0.0 | 0.4 ± 0.2 | 0.8 ± 0.2 | |
*
Significantly different (p ≤ 0.05) from CTL mice.
#
Significantly different (p ≤ 0.05) from WT mice.