Table 2. Semiquantitative analysis of immunohistochemistry in lung macrophages.
Lung sections prepared 3 d, 14 d and 28 d after administration of PBS (CTL) or nitrogen mustard (NM) to wild-type (WT) and FXR−/− mice were immunostained with antibodies to HO-1, COX-2, ARL11, STAT6, or CD163. Binding was visualized using a Vectastain kit. The number of macrophages positively staining for each antibody was quantified in 15 sections/mouse (original magnification 40×). Values are mean ± SE, n = 3–5 mice/treatment/time point. Data were analyzed by two-way ANOVA.
| Marker | WT |
FXR−/− |
|||
|---|---|---|---|---|---|
| CTL | NM | CTL | NM | ||
| 3 d | HO-1 | 21.0 ± 1.5 | 47.7 ± 0.7* | 61.3 ± 0.3# | 60.7 ± 2.9# |
| COX-2 | 5.0 ± 1.5 | 8.0 ± 2.1 | 7.7 ± 4.7 | 18.7 ± 1.8*,# | |
| ARL11 | 37.0 ± 2.5 | 38.7 ± 4.8 | 48.0 ± 3.2 | 44.0 ± 3.1 | |
| STAT6 | 45.7 ± 6.6 | 57.0 ± 8.0 | 48.0 ± 4.2 | 65.7 ± 11.6 | |
| CD163 | 29.0 ± 5.8 | 35.3 ± 4.8 | 26.0 ± 1.5 | 34.3 ± 1.8 | |
| 14 d | HO-1 | 8.0 ± 2.2 | 41.4 ± 6.5* | 21.4 ± 2.6 | 150.3 ± 6.5*,# |
| COX-2 | 13.8 ± 3.9 | 26.6 ± 6.4* | 22.2 ± 2.4 | 117.8 ± 6.1*,# | |
| ARL11 | 28.2 ± 4.4 | 59.4 ± 9.6* | 34.4 ± 1.7 | 102.4 ± 8.7*,# | |
| STAT6 | 31.0 ± 8.4 | 52.7 ± 9.0 | 40.0 ± 7.6 | 113.7 ± 18.8*,# | |
| CD163 | 4.6 ± 1.8 | 34.7 ± 4.9* | 14.2 ± 0.7 | 45.0 ± 13.5*,# | |
| 28 d | HO-1 | 24.0 ± 2.3 | 51.3 ± 2.2* | 13.0 ± 3.1# | 32.7 ± 2.0*,# |
| COX-2 | 30.0 ± 3.8 | 58.0 ± 6.4* | 20.7 ± 1.8 | 33.0 ± 5.2# | |
| ARL11 | 39.3 ± 2.8 | 54.3 ± 4.9 | 34.3 ± 5.4 | 54.7 ± 3.8* | |
| STAT6 | 58.7 ± 8.4 | 78.0 ± 12.7 | 42.0 ± 12.4 | 73.3 ± 3.8 | |
| CD163 | 38.3 ± 3.8 | 41.7 ± 9.4 | 20.3 ± 2.0 | 49.0 ± 3.2* | |
*
Significantly different (p ≤ 0.05) from CTL treated mice.
#
Significantly different (p ≤ 0.05) from WT mice.