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Antimitochondrial effect. (A) U1285 cells were treated for 24 h with increasing concentrations of the tested complexes or CCCP (3 μM). The percentage of cells with hypopolarized mitochondrial membrane potential was determined by Mito-ID® Membrane Potential Kit. ** p ≤ 0.01. Error bars indicate S.D. (B) TEM analysis. Transmission electron micrographs of untreated U1285 cells or 24 h treated cells with IC50 concentrations of complex 3 or complex 4.
