Figure 2.

Immunolocalization of endogenous UNC-84 and Ce-lamin in unc-84 and unc-83 mutant lines. (A) Embryos were double-stained by indirect immunofluorescence using antibodies against UNC-84 (left panels) and Ce-lamin (right panels) and viewed by confocal microscopy. Ce-lamin staining was detected in all nuclei and was not affected by the unc-84 mutation. Bar, 10 μm (all panels). (B) Summary of UNC-84 localization in all tested unc-84 alleles. Because unc-84(n322) is a small deletion that removes the antigen that the UNC-84 antibody was raised against, we expect a negative result. (C) The molecular lesions in tested alleles of unc-84. Class 1 mutations are written in black, class 2 mutations in dark gray, class 3 mutations in light gray, and class 4 mutations are boxed.