Figure 3.
Immunodetection of UNC-84 protein in unc-84, unc-83, and anc-1 mutant lines. Western blot analysis of mixed stages: wild-type (WT), UNC-84: GFP, unc-84(n369) (class 1), unc-84(n322) (class 4), unc-84(n1411) (class 4), unc-84(sa61) (class 3), unc-84(n399) (class 3), unc-83(n1408) and anc-1 lines. The positions of UNC-84 long (L, 125 kDa) and short (S, 98 kDa) protein isoforms in wild-type nematodes are indicated. A band corresponding in size to the UNC-84: GFP fusion protein is marked by an arrow.
