Figure 4.

Immunolocalization of UNC-84 during development. Animals at different stages of development were double-stained with antibodies against UNC-84 and Ce-lamin and viewed by confocal microscopy. UNC-84 was first detected at low levels at the 26-cell stage and was present in all cells (panels marked “embryonic development”). The UNC-84 signal became stronger as development progressed, and was present in all embryonic cells. UNC-84 was detected in most (but not all) cells at the L1/L2 larvae stages, whereas all cells stained positive for lamins, as expected (Liu et al., 2000). The UNC-84 and Ce-lamin signals colocalized at the nuclear envelopes of essentially all somatic adult cells, including the distal tip cell (DTC). The only adult cells that lacked UNC-84 signal were germ cells at the distal part of the gonad (g). Bar, 10 μm (each panel).