Figure 2.

The effects of Hydrogel-Tac local injection on the systemic immune system. At different time points, various lymphoid organs, such as blood, spleen, and LNs, were harvested from the mice treated with one subcutaneous injection of 0.1 mL of hydrogel or Hydrogel-Tac (2 mg/mL) and then analyzed by flow cytometry. LN-S refers to the LNs on the same side as the hydrogel injection and near the right hindlimb. LN-O refers to the LNs on the opposite side to the hydrogel injection and near the left hindlimb. (A) Lymphocyte, monocyte, and granulocyte subsets were analyzed between the hydrogel and Hydrogel-Tac groups. (B) CD3⁺, CD4⁺, and CD8⁺ T-cell subsets were examined between both groups. (C) Natural killer (NK1.1⁺, NK) and natural killer T (NK1.1⁺CD3⁺, NKT) cell subsets were determined between both groups. (D) CD19⁺ B and CD11c⁺ dendritic cell subsets were verified between both groups. (E) CD4⁺CD25⁺FoxP3⁺ regulatory T (Treg) cell subset was evaluated between both groups. Each group had three mice. Mean ± SD was used to represent the statistical data.