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Effect of ectopic expression of dominant negative Rac1, Cdc42, and RhoA on TGF-β1–induced actin reorganization. PC-3U cells were starved in 1% FBS for 12 h and stimulated with 10 ng/ml TGF-β1 for 30 min or 48 h before and after transient transfection with pRK5mycN17Rac1, pRK5mycN17Cdc42, or pRK5mycN19RhoA. Filamentous actin was visualized by TRITC-labeled phalloidin. Myc-tagged GTPases were visualized by a myc-specific antibody followed by FITC-labeled anti-mouse antibody. Bar, 20 μm.
