Figure 6.

Effects of TGF-β stimulation on GTP loading of Rac1, Cdc42, and RhoA. The amount of active, GTP-loaded Rac1, Cdc42, and RhoA was determined by GST pull-down assays with GST-PAK-CRIB, GST-WASP-CRIB, or GST-rhotekin, respectively, from TGF-β1–stimulated PC-3U cells. Rac1, Cdc42, and RhoA were detected by Western blotting by antibodies specific for the respective GTPase (A). Western blots were analyzed by densitometry and the data are combined into diagrams showing the activation of Rac1, Cdc42, and RhoA (B). Each column represents the means from three independent experiments.