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The uptake of 0.05 µM [3H]-adenosine in T. congolense IL3000. (A) Uptake over time, and (B) uptake at a fixed time point of 30 s, in the presence of unlabeled adenosine for the Km determination, using 0–1 mM adenosine in bloodstream forms of T. congolense. Graphs show one representative experiment out of two (panel A) or three (panel B) independent repeats, each carried out in triplicate. Error bars represent standard error of the mean (SEM).
