Figure 2.

Growth curve of L. mexicana Cas9, SUPKO, and SUPKO clones expressing T. congolense and T. vivax nucleoside transporters. Cells were seeded at density of 2 × 10⁵ cells/mL in HOMEM medium and counted manually in a Neubauer chamber viewed under microscope every 24 h. Graph was plotted using the average count of three independent determinations (Mean ± SEM; n = 3). When error bars are not shown they fall within the symbol.