Figure 5.

[³H]-adenosine (0.05 µM) uptake by TvxNT3 expressed in SUPKO. (A) Uptake over 120 s compared to control, and (B) Uptake in the presence of 0–1 mM unlabeled adenosine. (C) Adenosine uptake in SUPKO+TvxNT3 in the presence of unlabeled nucleosides and nucleobases. Purine and pyrimidine nucleobases and nucleosides at 100 µM competed with 0.05 µM of [³H]-adenosine for transport through TvxNT3. Figure shows the average of three independent experiments, each carried out in triplicate (% adenosine uptake average ± SEM, n = 3). Statistically significant difference from the control was determined by an unpaired, two-tailed t-test; *, p < 0.05; **, p < 0.01.