Figure 8.
The evaluation of CDC42 by WB and clonogenicity assay: (A) Western blot analysis of CDC42 expression in MDA-MB-231 cells before and after DOX treatment. The GAPDH antibody is used as a loading control. (B,C) The evaluation of clonogenicity after DOX treatment and CDC42 suppression. (B) Clonogenicity of MDA-MB-231 NT cells on day 23 after CDC42 inhibitor, ML141, treatment (100 cells seeded per well). (C) Three-fold decrease in the clonogenicity of MDA-MB-231 cells after DOX and CDC42 inhibitor, ML141, treatment (counted from ~1.5 × 106 cells initially seeded per flask) on day 23 after treatment evaluated in five independent experiments. * p < 0.05.