Table 2.
Antibodies used, their specificity, and source.
| Antibody Against | Description | Specificity/Immunogen | Concentration Used | Product No. and Manufacturer |
|---|---|---|---|---|
| AURORA B | Rabbit polyclonal | A peptide derived from within residues 1–100 of human Aurora B. | 1:300 | ab2254, Abcam, Cambridge, UK |
| ⍺-Tubulin | Mouse monoclonal | Recognizes an epitope located at the C-terminal end of the ⍺-tubulin isoform in a variety of organisms. | 1:1000 | T5168, Sigma-Aldrich, St. Louis, MO, USA |
| β-Actin | Mouse monoclonal | Synthetic peptide corresponding to human β-actin aa 1–100. | 1:500 1:2000 WB |
ab8226, Abcam, Cambridge, UK |
| CDC42 | Rabbit polyclonal | The details of the immunogen for this antibody are not available. | 1:100 1:500 WB |
ab187643, Abcam, Cambridge, UK |
| CDC42 | Mouse monoclonal | Specific for an epitope mapping between amino acids 166–182 at the C-terminus of CDC42 of human origin. | 1:50 | sc-8401, Santa Cruz, Dallas, TX, USA |
| CDX2 | Rabbit monoclonal | A synthetic peptide corresponding to residues near the N-terminus of human CDX2. | 1:50 | MA5-14494, Thermo Fisher Scientific, Rockford, IL, USA |
| EMI2 | Rabbit polyclonal | Recombinant protein corresponding to human EMI2. | 1:100 | PA5-55042, Invitrogen, Carlsbad, CA, USA |
| F-ACTIN | Phalloidin-iFlour 594 conjugate. | 1:500 | ab176757, Abcam, Cambridge, UK | |
| GAPDH | Mouse monoclonal | Raised against recombinant GAPDH of human origin. | 1:5000 WB | sc-47724, Santa Cruz, Dallas, TX, USA |
| γ-H2AX | Rabbit polyclonal | Recognizes human and mouse γ-H2AX. | 1:200 | 4411-PC-100, Trevigen, Gaithersburg, MD, USA |
| MOS (C237) | Rabbit polyclonal | Epitope mapping at the C-terminus. | 1:50 | sc-86, Santa Cruz, Dallas, TX, USA |
| NANOG | Mouse monoclonal | Recombinant human Nanog. | 1:50 | N3038, Sigma-Aldrich, St. Louis, MO, USA |
| OCT4 | Rabbit polyclonal | A peptide derived from within residues 300 to the C-terminus of human Oct4. | 1:200 | ab19857, Abcam, Cambridge, UK |
| p-AMPKα1/2 (Thr183/172) | Rabbit polyclonal | Epitope corresponding to phosphorylated Thr172 of AMPKα1 of human origin. | 1:50 | sc-101630, Santa Cruz, Dallas, TX, USA |
| pH3Ser10 | Mouse monoclonal | Recognizes phospho- S10 on histone H3. | 1:200 | ab14955, Abcam, Cambridge, UK |
| P62/SQSTM1 | Rabbit polyclonal | A synthetic peptide corresponding to human SQSTM1/ p62 (C-terminal). | 1:500 WB | ab91526, Abcam, Cambridge, UK |
| TEAD1 | Mouse monoclonal | Carrier-protein-conjugated synthetic peptide encompassing a sequence within the centre region of human TEAD1. |
1:100 | GT13112, Invitrogen, Carlsbad, CA, USA |
| YAP1 | Rabbit polyclonal | Recombinant YAP1 protein expressed in bacteria. |
1:400 | PA-46189, Invitrogen, Carlsbad, CA, USA |