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. 2023 Feb 14;10:rbad011. doi: 10.1093/rb/rbad011

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — In vitro supplementation with Na₂SeO₃ rescued the proliferation and osteogenic differentiation of OP-BMMSCs. The cells were treated with 10, 100 nM and 1 μM of Na₂SeO₃, while untreated cells served as the control (CTRL) group. (A) Proliferation of Na₂SeO₃-treated OP-BMMSCs was examined on Days 1, 3, 5 and 7 using CCK-8 assay, n = 8. (B) The gene expressions of osteoblast-specific markers in Na₂SeO₃-treated OP-BMMSCs were quantified with real-time RT-PCR, n = 4. (C) Representative images of mineralized extracellular matrix stained by ARS. Scale bar = 200 μm, n = 4. (D) Quantification of the stained mineral layers of Na₂SeO₃-treated OP-BMMSCs, n = 4. Values represent mean ± SEM. Statistically significant differences are indicated by * where P < 0.05 or ** where P < 0.01 between the indicated groups.