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. 2023 Feb 14;10:rbad011. doi: 10.1093/rb/rbad011

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Biocompatibility of modified bone cement and the GPx1 expression. OP-BMMSCs were cultured using the leached solution of SF/CPC and Se@SF/CPC, while cell culture medium without served as the CTRL group. (A) Cell viability was compared by a live/death staining assay. Scale bar = 500 μm. (B) Cell proliferation was evaluated by CCK-8 assay between the groups of the SF/CPC, Se@SF/CPC and CTRL, n = 6. (C) The Gpx1 gene expression of OP-BMMSCs was quantified with real-time RT-PCR, n = 4. (D) The GPx1 protein expression of OP-BMMSCs were detected by immunofluorescence assay. Scale bar: 100 μm. Values represent mean ± SEM. Statistically significant differences are indicated by * where P < 0.05 or ** where P < 0.01 between the indicated groups.