Table 7.
Karyogamy and spore formation in dlc1Δ and dhc1Δ zygotes Cells were sporulated in MEB-Gal at 26°C for 24 h.
| Straina | Karyogamyb | Spore formationc | Percentage of asci containingd
|
||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | >4 spores | |||
| Zygotic | |||||||
| Wild-type | 90 | 84 | 0 | 1 | 0.5 | 99 | 0 |
| dlc1Δ | 55 | 81 | 11 | 30 | 8 | 51 | 0 |
| dhc1Δ | 44 | 71 | 9 | 20 | 10 | 58 | 3 |
| kms1Δ | nde | 69 | 22 | 33 | 11 | 33 | 1 |
| dlc1Δ dhc1Δ | 3 | 47 | 49 | 33 | 10 | 7 | 0 |
| dlc1Δ kms1Δ | nd | 38 | 76 | 20 | 3 | 1 | 0 |
| dhc1Δ kms1Δ | nd | 68 | 28 | 27 | 16 | 29 | 0.5 |
| Azygotic | |||||||
| Wild-type | 81 | 0 | 0 | 1 | 99 | 0 | |
| dlc1Δ | 43 | 7 | 15 | 6 | 72 | 0 | |
| dhc1-d4Δ | 62 | 1 | 3 | 6 | 90 | 0 | |
| dlc1Δ dhc1-d4Δ | 27 | 23 | 32 | 13 | 31 | 0 | |
a
Strains used were Z310-10B (wild-type), F52-3A (dlc1Δ), F68-12B (dhc1-d4), Z121-4D (kms1Δ), F71-11D (dlc1Δ kms1Δ), F143-6B (dlc1Δ dhc1-d4), and F167-7B (kms1Δ dhc1-d4).
b
Percentage of binucleated conjugants, but only those without a meiosis I spindle.
c
Percentage of sporulated zygotes in total zygotes.
d
More than 200 sporulated cells were counted.
e
Not determined.