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RP-HPLC analyses of reaction mixtures upon the conjugation of 5′-p-dT7 with different amino ligands (Table 1). dT7: 5′-d(TTTTTTT); 5′-p-dT7: 5′-p-d(TTTTTTT); (1): MB-L6-NH-p-dT7; (4): CH≡C-CH2-NH-p-dT7; (5): Pyr-CH2-NH-p-dT7; (6): HO-(CH2)3-NH-p-dT7; (7): NH2-(CH2)6-NH-p-dT7; MB-L6-NH-p-, N-(6-aminohexyl)-4-methoxybenzamide residue; Pyr-CH2-NH–, pyrenemethylamine residue; -p-, -P(O)(OH)-; d(N), deoxyribonucleotide. See Section 3: Materials and Methods for details.
