Quantitative immunohistochemical analysis of specific markers in Z3, Z2, Z1 and PT in the hepatic parenchyma of squirrel monkeys (Saimiri spp.) infected with YFV. Circles represent areas expressing higher immunostaining cells in different hepatic areas. (A) Caspase 3 expression. (E) Immunostaining for Caspase 3 in hepatocytes (E-Z3), (E-Z2), (E-Z1) and bile duct (E-PT). (B) MLKL expression. (F) Immunostaining for MLKL in hepatocytes and inflammatory infiltrate (F-Z3), (F-Z2) inflammatory infiltrate, (F-Z1) in hepatocytes, (F-PT) in hepatocytes. (C) Expression of iNOS. (G) Immunostaining for iNOS in inflammatory infiltrate (G-Z3), (G-Z2) in hepatocytes, (G-Z1) hepatocytes, (G-PT) bile duct and inflammatory infiltrate. (D) Lysozyme Expression. (H) In inflammatory infiltrate (E-Z3), (E-Z2), (H-Z1) in Kupffer cells, (H-PT) in inflammatory infiltrate at 6 dpi.