Table 1.
List of the primers used for amplification of carbapenemase genes in the isolated Enterobacteriaceae.
| Target Gene | Amplified Product (bp) | Target Amplicon | |
|---|---|---|---|
|
blaKPC [17,18] F R |
5′-CGTTGACGCCCAATCC-3′ 5′-ACCGCTGGCAGCTGG-3′ |
390 | Class A serine enzymes |
|
blaIMP [17,18] F R |
5′-CATGGTTTGGTGGTTCTTG -3′ 5′-ATAATTTGGCGGACTTTGGC-3′ |
488 | Class B metallo-b-lactamases (MBLs) |
|
blaVIM [17,18] F R |
5′-ATTGGTCTATTTGACCGCGTC-3′ 5′-TGCTACTCAACGACTGAGCG-3′ |
780 | |
|
blaNDM-1 [19] F R |
5′-GGTTTGGCGATCTGGTTTTC-3′ 5′-CGGAATGGCTCATCACGATC-3′ |
621 | New Delhi metallo-b- lactamase-1 |