Table 3.

Mass assay of cytoplasmic and nuclear DAG in response to DMSO plus 1.5% ethanol  HL-60 cells were exposed to a combination of 1.25% DMSO and 1.5% ethanol for the indicated times. DAG mass was then assayed in both the cytoplasmic and nuclear fraction. The data are expressed as picomoles of DAG per milligram of protein. The data are the mean of three different experiments ± SD.

Stimulus	PLD activity
	Cytoplasm	Nucleus
None	127.6 ± 14.9	41.2 ± 5.7
5 min	117.8 ± 15.3	39.6 ± 6.1
10 min	123.5 ± 15.5	45.1 ± 5.6
15 min	NA	42.4 ± 4.5
20 min	NA	46.6 ± 4.7

NA, not assayed.