Table 4.
In vitro activity of nuclear PLD in the presence of various activators or inhibitors HL-60 cells were exposed to DMSO for 20 min and the nuclei where then isolated and assayed for PLD activity. The data are expressed as either the percentage of labeled nuclear phosphatidylethanol relative to total nuclear lipid radioactivity or picomoles per minute per milligram of protein of choline release (Martelli et al., 1999c). The data are the mean of three different experiments ± SD.
| Addition | PLD activity
|
|
|---|---|---|
| [3H]Palmitic acid labeling | Choline release | |
| None (basal activity) | 2.08 ± 0.18 | 3,257 ± 376 |
| PtdIns (4,5) P2 20 μM | 3.88 ± 0.39 | NA |
| ATP 30 μM | 2.57 ± 0.35 | 4124 ± 489 |
| Oleate 1 mM | 0.86 ± 0.092 | NA |
| GTP-γ-S 50 μM | 0.93 ± 0.012 | 1590 ± 256 |
| GDP-β-S 50 μM | 2.01 ± 0.19 | 3044 ± 338 |
NA, not assayed.