Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 21;18:949–970. doi: 10.2147/IJN.S399785

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Kuang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

MALAT1 competitively bound to miR-1914-3p to inhibit the activity of the TGFB1/SMAD3 signaling pathway and affect macrophage phagocytosis, apoptosis, and polarization. (A) MALAT1 expression in a normal medium or high glucose medium, determined by qRT-PCR. (B) MALAT1 expression in wound tissue from normal mice or diabetic mice, determined by qRT-PCR. (C) The correlation between MALAT1 and miR-1914-3p expression, measured in 31 mice skin by Spearman correlation analysis. (D) Predicted target miRNAs of MALAT1 by three online databases (ENCORI, DIANA-LncBase and RNA22). (E) Predicted binding sites between MALAT1 and miR-1914-3p by bioinformatics database. (F) Luciferase activity of MALAT1-WT and MALAT1-MUT detected by dual-luciferase reporter gene assay. (G) Relative enrichment of Ago2 by MALAT1 and miR-1914-3p detected by RIP assay. (H) Relative enrichment of MALAT1 detected by RNA pull-down. (I) MALAT1, miR-1914-3p and mRNA expression of MFGE8 expression, determined by qRT-PCR. (J and K) Uptake of DiI-labelled KCs-Exo by PMA-treated THP-1 cells, measured by flow cytometry analysis. (L and M) Apoptosis in PMA-treated THP-1 cells, measured by flow cytometry analysis. (N) mRNA expression of apoptosis-related factors (Bax and Bcl-2), macrophage polarization-related surface markers (iNOS, Arg-1 and CD206), TGFB1 and SMAD3, determined by qRT-PCR. (O and P) Protein expression of apoptosis-related factors (Bax and Bcl-2), macrophage polarization-related surface markers (iNOS, Arg-1 and CD206), MFGE8, TGFB1 and SMAD3, normalized to GAPDH, determined by Western blot analysis. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus the control group. Measurement data were expressed as mean ± SD. Comparison between two groups was conducted using independent sample t test. One-way ANOVA was used for data comparison among multiple groups, followed by Tukey’s post hoc test. The experiment was repeated independently three times.