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. 2023 Feb 14;24(4):3807. doi: 10.3390/ijms24043807

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Representative images and quantitative analysis of NADPH oxidase and the mRNA expression of NADPH oxidase subunits in STZ-induced diabetic mice with or without febuxostat treatment. (a) Representative immunoblots of NOX1, NOX2 and NOX4 expressions. (b) Quantitative analyses for NOX1/GAPDH, *** p < 0.001 vs. other groups. (c) Quantitative analyses for NOX2/GAPDH, * p < 0.05 and *** p < 0.001 vs. Cont, ^† p < 0.05 and ^††† p < 0.001 vs. Feb and ^‡ p < 0.05 vs. STZ group. (d) Quantitative analyses for NOX4/GAPDH, ** p < 0.01 vs. other groups. (e) NoxO1 mRNA, *** p < 0.001 vs. other groups. (f) p22phox mRNA, *** p < 0.001 vs. other groups. (g) p47phox mRNA, *** p < 0.001 vs. other groups. (h) p67phox mRNA, * p < 0.05 vs. Cont, ^†† p < 0.01 vs. Feb and ^‡ p < 0.05 vs. STZ group.