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. 2023 Feb 25;15(2):276–308. doi: 10.1007/s12393-023-09338-2

Table 3.

Summary of extraction of bioactive compounds obtained from tea under various PEF conditions

Sample Extraction conditions* b Keynote (s) Reference
Green tea 38.4 kV/cm, 20 °C, 160 µs, 667 pps, 2 µs Polyphenols and free amino acids

- A promising technology to maintain the quality of the bioactive compounds and color

- The inactivation of microorganisms (e.g., Escherichia coli and Staphylococcus aureus)

- Synergistic effect in the reduction of the microorganisms by the low-temperature storage and antibacterial property of polyphenols extracted

-No significant effect on polyphenol extraction with PEF (1.82 mg/mL (PEF) vs 1.82 mg /ml (untreated)) and 36% in amino acids compared to conventional methods

Zhao et al. [185]
Green tea 20–40 kV/cm, 121 °C, 50–200 µs, 667 pps, 2 µs Polyphenols, catechins, and free amino acids High retention of color and bioactive compounds Wang et al. [183]
Green tea infusions 38.4 kV/cm, 37 °C, 200 µs, 667 Hz, 2 µs NR - Shelf-life period more than 90 days (at 37 °C) for the infusions with PEF Zhao et al. [34]
Green tea infusions 20 to 40 kV/cm, 5–15 °C, 200 µs, 667 pps, 2 µs Catechins, polyphenols, and free amino acids

- Increased content of amino acids (specifically theanine) by 7.5% at 40 kV/cm with the loss of volatiles (≈10%)

- Efficient retention of the bioactive compounds and color

Zhao et al. [35]
Tea 0.9 kV/cm, 5 °C, 5 × 105 µs, 5 × 104 µs Polyphenols

- 27% maximum extraction yield for polyphenols

- Significant effects of the pulse and strength of electric field on the extraction yield

Zderic et al. [180]
Black tea 20 kV/cm, 95.83 µs, 125 Hz, 2 µs Total solids, polyphenols, and amino acids

- A 22.7% extraction yield for instant black tea powder

- Improved the tea solubility in cold water along with reducing the tea cream

- Total polyphenols (40.73 mg/g), amino acids (20.79 mg/g) and total solids (221.19 mg/g) were extracted using PEF

Ye et al. [186]
Unfermented Pu’er tea 18 kV/cm, 60 min (extraction time), 200 pps, 120 Hz, 0.3 µs Polyphenolics, and theine

- Improved the taste, aroma and other sensory parameters in tea samples compared with the effect of natural aging on teas

- Effective in the artificial aging as it improved the content of tea extract and its taste

- Shorter and quicker method for aging of unfermented Pu’er tea

- New method to enhance the tea quality and safety

- Total polyphenols extraction was ~ 30% and theanine ~ 4% and did not show significant difference compared to conventional method

Chen et al. [174]
Tea 0.9 kV/cm, 10 °C, 3 × 106 µs Polyphenols

- 27% maximum extraction yield for polyphenols

-Direct and positive relationship between the time of pulse and the treatment time

Zderic and Zondervan [33]
Tea 1.1 kV/cm, 100 µs, 0.1 × 10−3 s, 50 pulses Polyphenols Maximum extraction yield (32.5%) of polyphenols with the PEF without destroying bioactive compounds compared to the conventional hot-brewing method Zderic and Zondervan [179]

*1- Field strength (kV/cm), 2- Temperature (°C), 3- Pulse duration (µs), 4- Pulses per second (pps)/Frequency (Hz), 5- Pulse width (µs), NR not reported