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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Nat Genet. 2023 Jan 12;55(2):209–220. doi: 10.1038/s41588-022-01276-9

Figure 5. Single-nucleus transcriptomes reveal MCD gene enrichment in radial glia and excitatory neurons in the developing human cortex.

Figure 5.

(a) Uniform Manifold Approximation and Projection (UMAP) for single-nucleus transcriptome in 2nd-trimester fetal human telencephalon from a public dataset50. (b) UMAP enrichment patterns of an eigengene using MCD genes. Note enrichment for excitatory neurons and radial glia (dark brown). vRG: vertical radial glia, tRG: truncated radial glia, RG-div: dividing radial glia, oRG: outer radial glia, EN: excitatory neuron, nEN: newborn excitatory neuron, IPC: intermediate progenitor cell, STR: striatum, IN: interneuron, CTX: cortex, MGE: medial ganglionic eminence, CGE: central ganglionic eminence. (c) Quantification of enrichment of (b) based on cell types, showing enrichment for RG-div. (d) Four eigengenes decomposed from (b). (e) Quantification of enrichment of (d) based on cell types showing enrichment in dividing radial glia, microglia, and inhibitory cortical neurons from the medial ganglionic eminence (MGE). Net expression: Relative and scaled net expression level of a given eigengene generated by a defined gene list. The size and color of the dot plots in (c) and (e) indicate Pearson correlation coefficient r and corresponding non-adjusted asymptotic p-value derived from a two-sided Student’s t-test, respectively.