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. 2002 Mar;13(3):989–1000. doi: 10.1091/mbc.01-12-0581

Figure 6.

Figure 6

The actomyosin ring serves as a spatial landmark for Cps1p assembly at the division site. (A) cdc25-22 cells expressing GFP-Cps1p were synchronized at 36°C, released to 24°C in medium containing either 100 μM latrunculin A (LatA) or DMSO (solvent control), and stained with aniline blue. (B) Cells bearing cdc12-112, rng2-D5, or cdc15-140 were grown at nonpermissive temperature (36°C) and stained for DNA, microtubules (MT), and GFP-Cps1p with DAPI, TAT1 antibodies, and anti-GFP antibodies, respectively. Arrowheads and arrows indicate cells with microtubule configuration resembling postanaphase array and GFP-Cps1p medial structures, respectively.