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. 2023 Feb 16;24(4):4022. doi: 10.3390/ijms24044022

Figure 3.

Figure 3

Mechanical strain induced the apoptosis and anabolic/catabolic imbalance in chondrocytes through Piezo1. (a) Flow cytometry analysis of chondrocyte stained with Annexin V-FITC and PI (n = 3). (b) RT-qPCR analysis of BAX and BCL2 in chondrocytes (n = 3). (c) Protein level of BAX and BCL2 determined by Western blots in chondrocytes (n = 3). (d,e) RT-qPCR analysis of MMP3, MMP13, Aggrecan, and COL2A1 in chondrocytes (n = 3). (f,g) Representative immunofluorescence images of MMP3, MMP13, Aggrecan, and COL2A1 in chondrocytes (n = 3). (h) Quantified results of immunofluorescence. * p < 0.05 vs. NC; ** p < 0.01 vs. NC; # p < 0.05 vs. Yoda1; ## p < 0.01 vs. Yoda1. One-way ANOVA with Bonferroni’s test for (a–e,h), Kruskal–Wallis test with Dunn’s multiple comparisons test for COL2A1 in (d) and MMP3 in (e). Scale bar: 100 μm.