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. 2023 Feb 16;24(4):4022. doi: 10.3390/ijms24044022

Figure 5.

Figure 5

Blocking the signal transduction of the CaN/NFAT1 signaling axis protected chondrocytes from apoptosis. (a) Representative image of the Flou-4AM stain (n = 3), scale bar: 100 μm. (b) The activity of CaN is determined by the CaN Activity Kit (n = 3). (c) Representative immunofluorescence image of NFAT1 nuclear translocation (n = 3), scale bar: 10 μm. (d) Western blot analysis of NFAT1 in chondrocyte nucleoprotein (n = 3). (e) Quantified results of (d). (f,g) Flow cytometry analysis of chondrocyte stained with Annexin V-FITC and PI (n = 3). (h) mRNA levels of BAX and BCL2 in chondrocytes determined by RT-qPCR (n = 3). (i,j) Western blot analysis and quantified results of BAX and BCL2 (n = 3). ** p < 0.01 vs. NC; # p < 0.05 vs. strain; ## p < 0.01 vs. strain; ns, no significant differences. One-way ANOVA with Bonferroni’s test for (a,b,e,g,j), Kruskal–Wallis test with Dunn’s multiple comparisons test for (h). Scale bar: 100 μm.