Figure 3.

Cytochalasin D inhibits wound closure. (A and B) A cell undergoing closure of spontaneous wounds. Cytochalasin D (0.2 μM) was applied at the 6-min time point shown in C, and this causes the eventual cessation of hole closure and centripetal flow (10-min time point shown in D). The drug also induces a characteristic clearing of the cytoplasm and an accumulation of a phase-dense ring at the cell periphery. At the 12-min time point (E) the drug is washed out and the cell resumes hole closure and centripetal flow (22-min time point in F). Bar, 10 μm.