Figure 4.

Latrunculin A inhibits wound closure. (A–C) Closure of a hole produced by a microneedle (arrow in A) in a control cell; note the development of the characteristic phase-dense fringe (total elapsed time = 2 min). (D–F) Effect of latrunculin A perfusion immediately after the mechanical production of two new holes in the same cells shown in A–C. The holes were made and the drug was perfused ∼10 s before the capture of D. Note that the hole margins do not develop a phase-dense fringe and do not close, whereas the central cytoskeleton tends to undergo a retraction (total elapsed time = 10 min). Bar, 10 μm.