Table 2.
Primary and secondary antibodies used in this study.
| Molecular Marker | Role | Antibody | Antibody Type | Working Dilution | Source |
|---|---|---|---|---|---|
| Connexin43 | Target protein | Rabbit polyclonal | Primary | 1:2000 | Sigma Aldrich, St Louis, MO, USA #C6219 |
| GFAP-Cy3 | Müller cell and astrocyte marker | Mouse monoclonal | Primary | 1:1000 | Sigma Aldrich, #C9205 |
| Iba1 | Microglia marker | Rabbit monoclonal | Primary | 1:2000 | Abcam plc, Cambridge, UK #ab178846 |
| NLRP3 | Inflammasome marker | Goat polyclonal | Primary | 1:500 | Abcam plc, #ab4207 |
| Cleaved caspase-1 | Inflammasome marker | Rabbit polyclonal | Primary | 1:50 | Invitrogen, Auckland, New Zealand #PA5-38099 |
| Isolectin-B4-A594 | Blood vessel stain | Griffonia simplicifolia |
Primary | 1:100 | Molecular Probes, Eugene, OR, USA #I-21413 |
| PLVAP | Marker of leaky blood vessels | Mouse monoclonal | Primary | 1:1000 | Abcam plc, #ab27853 |
| NF-κB | Inflammation marker | Rabbit polyclonal | Primary | 1:1000 | Abcam plc, #ab16502 |
| Goat anti-rabbit Alexa Fluor 488 | - | Goat polyclonal | Secondary | 1:500 | Invitrogen, #A11034 |
| Donkey anti-rabbit Alexa Fluor 488 | - | Donkey polyclonal | Secondary | 1:500 | Invitrogen, #A21206 |
| Donkey anti-goat Cy3 | - | Donkey polyclonal | Secondary | 1:500 | Jackson Immuno Research Laboratories Inc., Westgrove, PA, USA #705-165-147 |