Table 2.
Relative stoichiometries of ODA-DC and outer dynein arm polypeptides The relative amounts of the outer dynein arm intermediate chains (IC78 and IC69) and of the Mr ∼70,000 and Mr ∼105,000 polypeptides of the ODA-DC were determined by quantitation of the Coomassie-blue stained SDS-polyacrylamide gel of sucrose density gradient fractions shown in Figure 1D. From these values and the masses predicted from the proteins' sequences (the mass of the Mr ∼70,000 polypeptide is predicted to be 62.2 kDa; see text), the molar ratios relative to IC78 were calculated.
| Polypeptide | Relative amounta | Mass (kDa) | Molar ratio |
|---|---|---|---|
| Mr∼105,000 | 1.00 | 83.4 | 0.92 (1) |
| IC78 | 1 | 76.5 | 1 (1) |
| IC69 | 0.87 | 63.4 | 1.05 (1) |
| Mr∼70,000 | 0.85 | 62.2 | 1.05 (1) |
a
Average of two lanes.