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Shuttling of cyclin A and cyclin E is insensitive to LMB. HeLa cells were fused and 20 nM LMB was added 40 min later. Nuclei were injected with 35 mg/ml cyclin A-GFP (A) or coinjected with 35 mg/ml cyclin E-GFP/CDK2K33R and 1 mg/ml Texas Red-conjugated dextran (MW 70,000) 20 min after addition of LMB (B). Fluorescence and DIC images were taken at the indicated time points after injection.
