Figure.

Supplemental Figure 8. LMB treatment of fused cells inhibits cyclin B1 export but not cyclin A shuttling. (A) HeLa cells were injected with an expression construct for cyclin B1-YFP and fused as described in MATERIALS AND METHODS 3 h later. Twenty minutes after fusion, 20 nM LMB was added to the cells. Fluorescence pictures were taken at different time points after LMB addition (top). (B) HeLa cells were fused, and after 20 min, 20 nM LMB was added. Cyclin A-GFP protein was injected into the nucleus 30 min after the addition of LMB, and fluorescence pictures were taken at different time points after injection.