Table 2.

Preparation of PCR Mix.

Component	1 × (µL)
Supermix	5
Reverse Transcriptase	2
DTT, 300 mM	1
Forward Primer, 10 µM	1.8
Reverse Primer, 10 µM	1.8
Probe, 2.5 µM	2
Water	5.4
Sample (80–117 ng)	1
Total	20