Table 3.
Thermal Cycler Conditions.
| Temperature, °C | Time | Number of Cycles |
|---|---|---|
| 50 | 60 min | 1 |
| 95 | 10 min | 1 |
| 95 | 30 s (ramp rate 2 °C/s) | 40 |
| 55 | 1 min (ramp rate 2 °C/s) | 40 |
| 98 | 10 min | 1 |
| 4 | Infinite | 1 |
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