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. 2023 Feb 6;24(4):3174. doi: 10.3390/ijms24043174

Figure 5.

Figure 5

The effects of three flavonoids on intracellular ROS level (A) and ATP content (B) under Dox treatment in H9c2 cells. Cells were pretreated with same concentrations of F-A, F-B, and F-C (10 µM) for 1 h, then exposed to Dox for 24 h. CCCP (carbonyl cyanide 3-chlorophenylhydrazone) as a negative contrast group. (A) The DCF fluorescence intensity in Dox group significantly increased compared to the NC group (p < 0.01). There was no marked change of DCF fluorescence intensity in the F-A, F-B, and F-C group without Dox. But F-A, F-B, and F-C can significantly inhibit the Dox-induced elevation of ROS level in comparison to the Dox model group (p < 0.01). (B) H9c2 cells treated with 2.5 μM Dox rapidly reduced the content of ATP. Compared to the NC group, F-A, F-B, and F-C with the absence of Dox has no significant decrease (p > 0.05) the content of ATP. The content of ATP compared to the Dox group was significantly increased by the administration of F-A, F-B, and F-C in the presence of 2.5 µM Dox. Results are mean ± SEM from three independent experiments. ## p < 0.01 vs. control, * p < 0.05, ** p < 0.01 vs. Dox group. ns, not significant.