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. 2002 Mar;13(3):1046–1057. doi: 10.1091/mbc.01-06-0315

Figure 1.

Figure 1

Purification of a squid nonmuscle myosin II. Coomassie-stained 8.5% SDS-PAGE of optic lobe homogenate (H), supernatants (S) and corresponding pellets (P) of squid optic lobe samples obtained by a series of four centrifugation steps (S1-P4). The final high-speed supernatant, S4 fraction, (indicated by asterisks) is enriched in a high molecular weight protein (p220). The ∼220 kDa protein (arrow) was further separated from other proteins by gel filtration of the S4 fraction in the presence of ATP (GF68). Molecular weight markers indicated by dashes on left: 200, 116, 98, 68, 31 kDa.