Table 3.

Quantitative analysis of nonmuscle myosin II in axoplasmic fractions

Sucrose fraction	Protein/ lane (μg)a	Proportion of total protein/lane (%)b	Proportion of total antigen/fraction (%)cd
			sqNMMII	NF
Supernatant	26.4	53.0	56.8	80.8
12%	21.3	42.8	0.0	9.1
15% (organelles)	1.3	2.6	43.2	10.1
45%	0.8	1.6	0.0	0.0
Total	49.8	100	100	100

^a Total protein in each gel lane for Coomassie stain and Western blot analysis (10 μl each sucrose fraction). 

^b Proportion of the total protein in each gel lane Coomassie stain and Western blot analysis. 

^c Percent of nonmuscle myosin II (sqNMMII) and neurofilament (NF) in sucrose density fractions. 

^d Percent of antigen/fraction has been adjusted to account for the total volume of each sucrose fraction.