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CAP15/17 are associated with microtubules. (A) Supernatants (S) and pellets (P) from 2 × 107 T. brucei procyclic (PF) or bloodstream (BF) forms treated by detergent and various NaCl concentrations were analyzed with anti-CAP17.A or anti-CAP15.A immune sera, respectively, and monoclonal anti-tubulin. This data shows that, after 200 mM NaCl, most of the CAP proteins disassociates from the subpellicular microtubules.
