Figure 3.

Summary of the different methods for characterization and quantification of EVs. The characterization and quantification of EVs are performed through different techniques such as nanoparticle tracking analysis (NTA), flow cytometry (FC), Western blot (WB), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). TEM analyzes EV diameters and morphology. Dynamic light scattering (DLS) determines EV size distribution and polydispersity by detecting the diffusion coefficient of the scattering EVs. NTA detects EV dimensions and concentrations through scattered light measurement and Brownian motion. RNA sequences and mass spectrometry permit cargo characterization. In particular, FC can be employed for size detection, particle concentration, and EV-specific markers. Tunable resistive pulse sensing (TRPS) only measures particle concentration. Created in Biorender.com, accessed on 6 December 2022.